Ear Cytology

MANUALLY

• You should label the slide with the name of the pet on the short side, and the cells should be placed L on top, R underneath

• The swabs should be rolled across the slide in order to prevent cell damage and should be passed through a flame on the underside for a second or two to fix the cells (this is particularly helpful with excess ear wax cases)

• Take the fixative labeled Ears and begin to dip - use a clothespin to help with grip.

• Blue stain - 20 seconds , Red stain - 20 seconds, Purple stain - 20 seconds

• After the slide is stained and dried (can use a kim wipe to BLOT dry the slide), it is placed on the stage of the microscope and read on the high and dry setting.